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Biochemical validation and analysis of S. pneumoniae FakB proteins. A, purity of SpFakA and the three FakB proteins assessed by gel electrophoresis and Coomassie-staining. B, dependence of FA kinase activity on FakB concentration in the biochemical assays using 20 μm [14C]FA (SpFakB1, [14C]16:0; SpFakB2, [14C]18:1; and SpFakB3, [14C]18:2) and 0.2 μm SpFakA.
