Figure 1.

Low-molecular-weight haloalkanes similar to the HaloTag ligand are well-tolerated by cultured cells. A, structures of the reactive linker of HaloTag ligands and the four similar chemicals that were tested. B and C, lethal dose curves were generated following 24-h incubation of haloalkanes at the indicated concentrations in COS-1 cells (B) and primary hippocampal neuronal cultures (C) using the CellTiter Blue cell viability assay. Shown are data from one representative experiment with seven (COS-1) or three to six (neurons) technical replicates (means ± S.D.) that was repeated three times for COS-1 cells and twice for hippocampal cultures.