Figure 4.
Structural model of the BBS2-7-9 complex. A, three orthogonal views of the IMP localization densities computed for each subunit. The ribbon structure of the centroid model is depicted. B, inter-domain and inter-protein cross-links mapped onto the centroid model ribbon structure. Some domains are labeled to provide landmarks. C, distributions of Euclidean Cα distances of the cross-linked lysines for each cross-linker. Blue bars represent cross-links within the expected distance constraints and orange bars represent cross-links outside the expected constraints (35 Å for DSS, 31 Å for DSG, and 33 Å for Leiker). D, the same views as in A docked in the EM 3D reconstruction of BBS2-7-9 complex. The reconstruction is depicted as a gray mesh surface.
