Table 1.
Identified protamines
Asterisk indicates the di-aminopropanylated C terminus. The lower part of the table corresponds to protamines of lower abundance. Amino acids in parentheses do not contain fragment ions confirming their location. These residues can be interchanged within the parentheses.
| M + H | Sequence | Protein name | First amino acid residue no. | Last amino acid residue no. | NCBI accession no. |
|---|---|---|---|---|---|
| 3955.50 | RRRRRRKSRRRRRRRRRRSKGSRSPRRR* | PL-1 (MpPRM) | 182 | 209 | BAU71552 |
| 3799.41 | RRRRRRKSRRRRRRRRRRSKGSRSPRR* | PL-1 (MpPRM) | 182 | 208 | BAU71552 |
| 3942.56 | RRRRRRRHHKKGKKKGRRRRRKRRRGRR* | PL-1 (MpPRM) | 102 | 129 | BAU71552 |
| 3313.16 | RRRRRRRRRRRRRRSRRRRGRK* | PL-1 (MpPRM) | 146 | 167 | BAU71552 |
| 7466.75 | RRRRRRRHGRKGKKPKRHRRRRRRRRRGRRKAHRGRKKKRG-GRRKGKRRHRRRRR* | PL-3 | 162 | 216 | PTQ32223 |
| 4082.51 | RSTSRSRSRGRRRRRRRHRRRRGRRRRGRK* | PL-2 | 207 | 236 | PTQ35141 |
| 3780.36 | RRRRRRKSRRRRRRRRRR(SKGSH)SPRR* | PL-1 (MpPRM) | 182 | 208 | BAU71552 |
| 4139.33 | RRRRRRKSRRRR(RRTVDRRRRRRRRRRR) | PL-1 (MpPRM) | 182 | 209 | BAU71552 |
| 4366.65 | RRRRRRKSRRRRRRRRRRSKGSRSPRRRHSSR | PL-1 (MpPRM) | 182 | 213 | BAU71552 |
| 7819.01 | RRRRRRRHGRKGKKPKRHRRRRRRRRRGRRKAHRGRKKKRGGRRKGKRRHRRRRR(GKSH) | PL-3 | 162 | 220 | PTQ32223 |