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. 2019 Sep 17;294(44):16400–16415. doi: 10.1074/jbc.RA119.010628

Figure 8.

Figure 8.

α-GalNAcase GH31s cleave α-GalNAc from the peptide backbone. a, a peptide containing a GalNAc-α-threonine residue (BODIPY-IL29) was subjected to overnight enzymatic cleavage by the GH31 exo-α-GalNAcases from B. caccae and B. plebeius identified in this study. Cleavage products were then separated and analyzed via HPAEC-PAD. b, the cleavage of GalNAcylated BODIPY-IL29 was additionally followed via TLC at 5 and 45 min.