Table 1.

Substrate specificity of the GH31s and GH109 examined in this study

0.1 mm substrates were incubated at 37 °C with 0.5 mg/ml of the enzymes in 50 mm NaH₂PO₄, pH 7.5, for at least 30 min. NT, not tested.

Substrate	tBpGH31/BpGH31	tBcGH31/BcGH31	tCpGH31	BvGH109_1
GalNAc-α-MU	Yes	Yes	Yes	Yes
GalNAc-α-pNP	Yes	Yes	NT	Yes
Gal-α-MU	No	No	No	No
Glc-α-MU	No	No	No	No
Man-α-MU	No	No	NT	No
Xyl-α-pNP	No	No	NT	No
A-antigen Type2tetra-MU	No	No	NT	Yes