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. 2019 Sep 13;294(44):16309–16319. doi: 10.1074/jbc.RA119.009621

Figure 3.

Figure 3.

SyFtsZ assembly in buffers of different concentration of KAc and MgAc. A–C, assembly kinetics of 5 μm SyFtsZ in 5 mm MgAc and different concentrations of KAc, from 0 to 350 mm (A); in 100 mm KAc and different concentrations of MgAc, from 0 to 10 mm (B); and in 2.5 mm MgAc and different concentrations of KAc, from 0 to 350 mm (C). D–E, negative-stain EM images of 5 μm SyFtsZ assembled in 5 mm MgAc, 350 mm KAc (D); in 10 mm MgAc, 100 mm KAc (E); and in 2.5 mm MgAc, 100 mm KAc (F). Bar, 200 nm. All buffers contain 50 mm HEPES, pH 7.5, and 0.5 mm GTP.