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. 2019 Sep 13;294(44):16309–16319. doi: 10.1074/jbc.RA119.009621

Figure 5.

Figure 5.

A–C, SyFtsZ minirings observed by negative-stain EM. A, 10 μm SyFtsZ in HMK buffer at pH 7.5 assembled in the presence of 0.5 mm GTP. In addition to straight bundles and toroids, some minirings are also observed. The diameter of miniring is ∼20 nm. Bar, 100 nm. B and C, 10 μm SyFtsZ assembled into oligomers and minirings in the presence of 0.5 mm GDP at pH 7.5 (B) or at pH 6.5 (C). Bar, 100 nm. Arrows point to minirings. D and E, SDS-PAGE analysis of sedimentation of 5 or 10 μm SyFtsZ with 1 mm GTP or 1 mm GDP centrifuged at 50,000 rpm (D) or 90,000 rpm (E) for 30 min in a Beckman TLA100 rotor. Numbers in parentheses indicate the percentage of protein in the pellet.