Figure 6.
Cosedimentation binding data for N-terminal cMyBP-C with unlabeled actin at 5 μm. Although binding data used for TPA analysis were done using phalloidin-stabilized ErIA-labeled F-actin at 1 μm, here nonstabilized and unlabeled F-actin at 5 μm (final concentration) is used to demonstrate that binding of N-terminal cMyBP-C (0–40 μm) with actin under these conditions is consistent with earlier results by others (9, 22, 23). A–C, comparison of C0–C2 (in red in each panel) with modifications and mutations that were used in Figs. 2, 3, and 4, respectively. D, apparent dissociation constants (Kd) and molar binding ratios (Bmax) for binding of recombinant N-terminal cMyBP-C to unlabeled F-actin. Data represent mean ± S.E. Significant difference in Kd or Bmax relative to human C0–C2 (*, p < 0.01; **, p < 0.005). Error bars denote ± S.E.