Table 1.
TPA-derived parameters at saturation of effect
Data represent mean ± S.E. (n > 4). All bound cMyBP-C significantly reduced amplitude (p ≤ 0.0001) and increased the combined rate (p ≤ 0.003) at saturation as compared to actin alone. Significant difference in amplitude or combined rate relative to C0–C2 (*, p ≤ 0.03; **, p ≤ 0.0005; ***, p ≤ 0.0001) or C0–C1 (#, p ≤ 0.001). Significant difference in correlation times, φ1 or φ2, as compared to actin alone (#, p < 0.03). Saturation of correlation times were determined as the average of all concentrations, but excluding the points denoted by arrows in Fig. 5. Note that for two exponential fits the weighting (r1, r2) of the correlation times (φ1, φ2) does not differ between actin alone and C0–C2 binding, where r1 ∼0.07 and r2 ∼0.04.
| cMyBP-C | Amplitude | φ1 | φ2 | Combined rate |
|---|---|---|---|---|
| ° | μs | μs | radians/ms | |
| Actin alone | 58 ± 1 | 19 ± 1 | 179 ± 10 | 13 ± 1 |
| C0–C2 | 36 ± 1 | 17 ± 1 | 167 ± 11 | 71 ± 4 |
| C0–C2+PKA | 46 ± 1***# | 15 ± 1# | 165 ± 16 | 29 ± 6** |
| C0–C2 3SD | 40 ± 1* | 18 ± 1 | 184 ± 13 | 70 ± 4 |
| C0–C2 S133D | 33 ± 1 | 23 ± 1# | 157 ± 14 | 59 ± 1* |
| C0–C1 | 39 ± 1 | 16 ± 1 | 153 ± 12 | 57 ± 3* |