Figure 1.

Disrupted T lymphopoiesis in ikzf1^{Δ4 + 3/Δ4 + 3} mutant larvae. A, the target sites for ikzf1 gene editing. The AlwI restriction enzyme was used to identify one mutant allele (purple). The AGG and GGG sequences (red) are the protospacer adjacent motifs. Red triangles indicate the mutation sites in two mutant alleles. B, the ikzf1^{Δ4 + 3} and ikzf1⁺¹ mutations lead to generation of truncated Ikzf1 proteins lacking zinc finger domains. The ikzf1^{Δ4 + 3} mutation (in amino acid (aa) 28) and ikzf1⁺¹ mutation (in aa 398) caused formation of 57- and 403-aa truncated proteins, respectively. The purple squares indicate the mismatched amino acid caused by the frameshift in the mutant compared with the WT. C, whole-mount in situ hybridization (WISH) of ccr9a, ccr9b, lck, and rag1 in the thymus of ikzf1^{Δ4 + 3/Δ4 + 3} and their siblings (Sib). The values in the bottom right corner of each panel indicate counts with a typical appearance as presented (first number) in the total number of examined samples (last number). The red circles indicate the thymi.