Figure 5.

SORD contributes to intracellular erythritol in A549 lung cancer cells. Validation of gene expression knockdown, intracellular erythritol levels, and erythritol labeling from [U-¹³C]glucose. mRNA levels were determined by quantitative real-time PCR for (a) ADH1C and (b) SORD, (c) resulting intracellular erythritol levels represented by the measurement of the derivative erythritol_4TMS: signal intensity was normalized to internal standard (IS) d₆-pentanedioic acid and live cell number. d, MID of erythritol_4TMS (mean ± S.E.) after 30 h of [U-¹³C]glucose labeling in A549 lung cancer cells, extraction and derivatization; obtained p values compared with siCtrl using Welch's t test and Bonferroni correction (n = 5) to adjust for multiple comparison: *, p < 0.05; **, p < 0.01; ***, p < 0.001 (n = 4, representative results).