Figure 7.

Dynamics of β2 is not influenced by N-glycosylation. MDCK cells were transiently transfected with the SCN2B-yfp vector to express WT or fully unglycosylated (ung) β2 and grown for 2 days on glass supports. Mobility of β2-YFP on three different cellular locations was monitored by FRAP with a confocal microscope. Line charts of fluorescence intensity (mean ± S.D. (error bars)) of at least three representative experiments show comparable mobile fraction between β2 WT (blue line) and mutant (red line) at the three regions analyzed (i.e. the cell end (A), the cytoplasm matrix (B), and in large vesicular structures (C)). For each, images on the right show a representative cell prebleached, just after bleaching, and after fluorescence recovery (arrowheads mark the bleached area); see the complete FRAP data in Table S1. Scale bar, 10 μm.