Figure 1.

Alignment of KimA homologs and domain organization of the KimA^Lmo protein. A, MUSCLE alignment of the KimA^Bsu, KimA^Lmo, and KimA^Sau homologs from B. subtilis, L. monocytogenes (Lmo2130), and S. aureus (Sacol2443), respectively, generated with the Geneious software package (63). Amino acids in black, gray, and white have an amino acid similarity of >80, 60–80, or <60%, respectively. B, predicted membrane topology of KimA^Lmo overlaid with a MUSCLE alignment between KimA^Bsu and KimA^Lmo. The dashed line indicates the position at which the KimA^Lmo protein was truncated. Amino acids in black are identical; amino acids in gray are similar, and amino acids in white are nonsimilar.