Figure 4.

Potassium-dependent growth of E. coli synthesizing the full-length and the C-terminally truncated KimA^Lmo protein. E. coli LB650 strains harboring plasmids pWH844 (empty plasmid), pBP384 (kimA^Lmo), and pBP396 (kimA^Lmo ΔC terminus) were grown to an OD₆₀₀ of 0.3–0.5 in M9 minimal medium supplemented with 50 mm KCl. The cells were washed for 1 h in potassium-free M9 medium. Multiwell plate reader growth assays with different KCl concentrations were performed (n = 4). The growth rates were plotted against the KCl concentrations and fitted to the Michaelis–Menten equation. Bars, means; dashed lines, fitted curves. Significant differences between the fitted curves are shown (p < 0.0001 (****)) (F_(4,2307) = 540.1, one-way ANOVA with Tukey's post hoc test).