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. 2019 Sep 9;294(44):16020–16033. doi: 10.1074/jbc.RA119.010046

Figure 8.

Figure 8.

Interaction between c-di-AMP and potassium transporters determined by DRaCALA. A, autoradiographs showing the interaction between [32P]c-di-AMP and the potassium transporters as well as the truncated variants that are present in whole-cell lysates of the E. coli strains Rosetta (DE3) carrying the plasmid pGP172 (empty plasmid) or the derivatives pBP265 (kimALmo), pBP266 (kimALmo ΔC terminus), pBP267 (kimASau), or NEB T7 Express Iq carrying the plasmid pWH844 (empty plasmid) or the derivatives pBP346 (kimALmo C-terminal domain), pBP347 (kimASau C-terminal domain), pBP345 (ktrCLmo), pBP559 (kdpABCLmo), and pBP560 (kdpDLmo). Both empty vectors showed similar nonbinding (data not shown). B, fraction bound of [32P]c-di-AMP is shown for lysates from E. coli induced overnight for the expression of the indicated gene. Bars, means (n > 3). p < 0.01 (**), p < 0.001 (***), and p < 0.0001 (****) compared with empty plasmid alone (F(8,20) = 275.9, ANOVA with Dunnett's post hoc test).