Table 2.
Strains
| Name | Genotype | Description | Reference |
|---|---|---|---|
| E. coli | |||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F' proAB lacIq ZΔM15 Tn10 (Tetr) ] | Cloning | Stratagene |
| LB650 | F− thi lacZ gal rha kup1 (trkD1) ΔkdpABC5 ΔtrkH (Cmr) ΔtrkG (Kanr) | Potassium uptake studies | Ref. 54 |
| LB2003 | F− aroE rpsL metE thi gal rha kup1 (trkD1) ΔkdpABC5 ΔtrkA aroE+ | Potassium uptake studies | Ref. 54 |
| Rosetta (DE3) | F− ompT hsdSB(rB− mB−) gal dcm (DE3) pRARE (Cmr) | Protein expression | Novagen |
| NEB T7 Express Iq | MiniF lacIq(CamR)/fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10–TetS)2 [dcm] R(zgb-210::Tn10–TetS) endA1 Δ(mcrC-mrr)114::IS10 | Protein expression and DRaCALA | New England Biolabs |
| B. subtilis | |||
| 168 | trpC2 | WT | Laboratory collection |
| L. monocytogenes | |||
| EGD-e | WT | Serotype 1/2a strain | Laboratory collection |
| BPL77 | ΔcdaA | L. monocytogenes strain without a DAC | This work |