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TA biosynthetic pathway from the C4/C5 cleavage of Asc. The carbon atoms in each molecule were indexed according to the numbering in Asc. VvL-IdnDH catalyzing the conversion of l-idonic acid to 5-keto-d-gluconic acid was characterized previously by DeBolt et al. (3). Vv2KGR responsible for the conversion of 2-keto-l-gulonic acid to l-idonic acid was identified in the present study.
