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Identification of Vv2KGR enzymatic assay products. A–D, chromatograms for authentic 2-ketogulonic acid standard (A), enzyme assay product with denatured recombinant enzyme (B), authentic l-idonic acid standard (C), and enzyme assay product with intact recombinant enzyme (D). The main peak for l-idonic acid was detected at 6.43 min, and the m/z values are given for authentic l-idonic acid standard (E) and enzyme assay product (F).
