Table 2.
Enzymatic activity of Vv2KGR with different substrates
Each enzymatic unit was defined as the amount of the enzyme that catalyzes the conversion of 1 μmol of substrate/min under the tested assay condition. −, no activity.
| Substrate | Coenzyme | Km | Vmax | Kcat |
|---|---|---|---|---|
| mm | units/mg | s−1 | ||
| d-Glyoxylate | NADH | 0.248 | 69.380 | 192.10 |
| NADPH | 0.044 | 13.820 | 38.26 | |
| Pyruvate | NADH | 3.453 | 0.442 | 0.01517 |
| NADPH | 1.371 | 0.548 | 0.01223 | |
| Hydroxypyruvate | NADH | − | − | − |
| NADPH | 0.096 | 6.295 | 6.973 | |
| 2KLG | NADH | 1.561 | 17.190 | 9.520 |
| NADPH | 0.700 | 7.544 | 4.178 |