Figure 5.

UL33 promotes tumorigenesis in U251 malignant glioma cells. A, U251 iUL33 and U251 iUS28 cells were cultured as 3D spheroids, and the effect of receptor expression was assessed. The graph depicts pooled data of three individual experiments performed in octuplicate, presented as mean ± S.D. (error bars). B, orthotopic glioblastoma model in which U251-FM iUL33, U251-FM iUS28, or U251-FM cells were injected in the striatum of mice. Mice were fed doxycycline in their drinking water to induce UL33 and US28 expression. Tumor size was measured via bioluminescence imaging (BLI) in six mice per cell line. Data are depicted as mean ± S.E. (error bars). C, survival of mice in the in vivo study. *, p < 0.05; **, p < 0.01; and ****, p < 0.0001.