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KLHL9/KLHL13/CUL3 E3 Ub ligase complex components are novel IRS1 interactors up-regulated by ATG16L1 deficiency. A, co-immunoprecipitation of 3xHA-KLHL9, KLHL13-HA, and CUL3-HA with IRS1myc or the myc epitope alone in HEK293T cells. B, Klhl9, Klhl13, and Cul3 mRNA expression was examined in WT and ATG16L1 KO MEFs by qPCR (n = 3–4). p value was calculated using a two-tailed paired t test. *, p < 0.05; **, p < 0.01.
