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. 2019 Sep 12;294(44):16172–16185. doi: 10.1074/jbc.RA119.009110

Figure 8.

Figure 8.

Adipose tissue IRS1 protein content and Atg16l1, Klhl9, Klhl13, and Cul3 gene expression is altered upon high-fat feeding and in obesity. A, Atg16l1 mRNA expression was examined by qPCR in eWAT of LFD and HFD mice (n = 3/group). B, IRS1 protein content was analyzed by immunoblotting in epididymal white adipose tissue (eWAT) of LFD and HFD mice (n = 8/group). p value was calculated using a two-tailed unpaired Mann-Whitney t test. C, Klhl9, Klhl13, and Cul3 mRNA expression was examined by qPCR in eWAT of LFD and HFD mice (n = 3/group). p value was calculated using a two-tailed unpaired t test. *, p < 0.05. D, correlation analysis of BMI and ATG16L1, KLHL9, KLHL13, or CUL3 gene expression in publicly available datasets of human adipose tissue.