Table 1.

KLHL9 BioID

Expression of FLAGBirA*–KLHL9 or FLAGBirA* negative control from integrated cassettes in Flp-In 293 T-REx cells was induced with tetracycline in the presence of 50 μm biotin for 24 h. Biotinylated proteins were purified on streptavidin-Sepharose beads and recovered proteins were identified by LC-MS/MS. Total spectral counts for two independent experiments and two technical replicates are shown for the high-confidence interactors CUL3, KLHL13, and IRS1 (based on Bayesian False Discovery Rate (BFDR) <0.01), in comparison with 20 runs of FLAGBirA* negative control.

Gene	Control peptide counts	Peptide counts	Sum	BFDR
Cul3	3 0 0 0 0 3 0 0 0 0 0 0 0 0 2 0 0 0 0 0	153 149 162 147	611	0
Klhl13	0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	110 110 110 110	440	0
Irs1	0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	12 11 16 11	50	0