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. 2019 Sep 18;294(44):16429–16439. doi: 10.1074/jbc.RA119.009006

Figure 5.

Figure 5.

USP17 knockdown not only decreases H4K20 monomethylation but also increases p21 levels. A, B, and G, MCF7 cells were transiently transfected with the indicated siRNAs. After 72 h, an immunoblot (IB) analysis was performed with the indicated antibodies. Ctrl, control. C, H1299 cells were transfected with the indicated constructs and siRNAs. The cell lysates were analyzed by immunoblotting with the indicated antibodies. D and E, MCF7 cells were transiently transfected with the indicated siRNAs. After 72 h, a FACS analysis was performed (D). The percentages of cells in each phase of the cell cycle are indicated (E). Data are the means and standard deviations of three independent experiments. F, MCF7 cells were transiently transfected with the indicated siRNAs. After 72 h, cell viability was measured by a WST-8 cell proliferation assay. Data are the means and standard deviations of three independent experiments. **, p < 0.01. H, a proposed model illustrating how USP17 induces SET8 protein stabilization through deubiquitination.