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. 2019 Sep 21;9(10):244. doi: 10.3390/brainsci9100244

Figure 2.

Figure 2

The effects of acute nicotine on anxiety-like behaviors in B6J and B6N substrains. In the B6J strain, mice receiving a lower dose of nicotine (0.05 mg/kg) spent more time in the open arms of the elevated plus maze than mice receiving higher doses (0.1 and 0.25 mg/kg), indicative of anxiolytic-like behavior. In stark contrast, B6N mice show no preference for the open arms of the EPM at 0.05 or 0.1 mg/kg. Only at the highest dose (0.25 mg/kg) do they show a significant increase in the time spent in the open arm of the EPM. There was no effect of nicotine on the total numbers of crosses between arms to suggest non-specific effects of nicotine on locomotion. The data are reported as mean + SE. Each data point is representative of the averaged mouse response at the given dose. Significant difference is based on Holm-Sidak post hoc test following two-way ANOVA between both substrains (**** p < 0.0001), n = 8/group.