No genetic interactions between Snmp1 and OS-E/F mutants. (A) Representative 5-sec traces from wild-type, Snmp1 mutants, OS-E/F double mutants, or Snmp1/OS-E/F triple mutants in response to 100% farnesol. (B) Time constants of deactivation with the genotypes indicated: wild-type = 165.16 ± 27.1 msec, Snmp1 mutant = 396.7 ± 58.9 msec, OS-E/F mutant = 882.31 ± 121.92 msec, and Snmp1/OS-E/F mutant = 768.31 ± 88.7 msec. ANOVA P-values: wild-type to Snmp1 mutant = 0.0031; wild-type to OS-E/F mutant = 9.27 × 10−5; wild-type to Snmp1/OS-E/F triple mutant = 2.91 × 10−5; Snmp1 mutant to OS-E/F mutant = 0.006; Snmp1 mutant to Snmp1/OS-E/F mutant = 0.0064; and OS-E/F mutant to Snmp1/OS-E/F mutant = 0.46. n = 6–7 for each genotype. Error bars indicate SEM. All P-values were determined by one-way ANOVA with post hoc Tukey’s test.