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. 2019 Sep 4;213(3):911–922. doi: 10.1534/genetics.119.302558

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Copyright © 2019 Durdevic and Ephrussi

Available freely online through the author-supported open access option.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Localization of Aub and Ago3 in the egg chamber and embryos depends on Vasa. (A) Box plot showing percentage of oocytes and embryo progeny of wild-type (w¹¹¹⁸), vas^PD/D1, vas^PD/D1; vas-Gal4 > GFP-Vas^WT, vas^PD/D1; vas-Gal4 > GFP-Vas^DQAD, vas^PD/D1; vas-Gal4 > GFP-Vas^GNT, and vas^D1/D1; vas-Gal4 > GFP-Vas^WT flies displaying Aub-positive pole plasm, as determined by immunohistochemical detection of Aub. Experiments were performed in three independent replicates. (B) Box plot representing percentage of oocytes and embryo progeny of wild-type (w¹¹¹⁸), vas^PD/D1, vas^PD/D1; vas-Gal4 > GFP-Vas^WT, vas^PD/D1; vas-Gal4 > GFP-Vas^DQAD, vas^PD/D1; vas-Gal4 > GFP-Vas^GNT, and vas^D1/D1; vas-Gal4 > GFP-Vas^WT flies displaying Ago3-positive pole plasm, as determined by immunohistochemical detection of Ago3 protein. Experiments were performed in three independent replicates. (C) Quantitative PCR analysis for LTR transposons burdock, blood, and gypsy and non-LTR transposon HeT-A in vas^PD/D1, vas^PD/D1; vas-Gal4 > GFP-Vas^WT, vas^PD/D1; vas-Gal4 > GFP-Vas^DQAD, vas^PD/D1; vas-Gal4 > GFP-Vas^GNT, vas^PD/D1, vas^PD/D1; vas-Gal4 > GFP-Vas^WT, vas^PD/D1; vas-Gal4 > GFP-Vas^DQAD, and vas^PD/D1; vas-Gal4 > GFP-Vas^GNT flies. Expression of transposons in wild-type (w¹¹¹⁸) was set to 1 and normalized to rp49 mRNA in individual experiments. Error bars represent SD from three biological replicates.