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. 2019 Sep 4;213(3):911–922. doi: 10.1534/genetics.119.302558

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Copyright © 2019 Durdevic and Ephrussi

Available freely online through the author-supported open access option.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Vasa activity in the germarium is essential for germ cell development. (A) Box plot representing length of ovaries of 3-, 10-, and 20-day-old wild-type (w¹¹¹⁸), vas^D1/D1, vas^D1/D1; vas-Gal4 > GFP-Vas^WT, and vas^D1/D1; matTub-Gal4 > GFP-Vas^WT flies. The measurements were performed on 10 flies (n = 10). (B) Box plot representing the number of egg chamber–producing ovarioles per 3-, 7-, 10-, 15-, and 20-day-old wild-type (w¹¹¹⁸), vas^D1/D1, vas^D1/D1; vas-Gal4 > GFP-Vas^WT, and vas^D1/D1; matTub-Gal4 > GFP-Vas^WT females. Experiment was performed on five flies (n = 5). (C) Egg-laying rate (right panel) and hatching rate (left panel) measured daily between day 3 and day 20 postpupal enclosure of wild-type (w¹¹¹⁸), vas^D1/D1, vas^D1/D1; vas-Gal4 > GFP-Vas^WT, and vas^D1/D1; matTub-Gal4 > GFP-Vas^WT females. Experiments were performed in five independent replicates.