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. 2019 Sep 4;213(3):849–863. doi: 10.1534/genetics.119.302525

Figure 1.

Figure 1

tRNASer with a proline UGG anticodon and various secondary mutations allow nonlethal levels of mistranslation. (A) Mass spectrometry analysis of the cellular proteome was performed on wild-type strain (BY4742) containing either wild-type tRNASer, tRNASerUGG-G9A or tRNASerUGG-G26A. Mistranslation of serine at proline codons was quantified at all four proline codons. (B) Growth rates for each strain in A were determined from growth curves of the strains diluted to an OD600 of ∼0.1 in media lacking uracil and grown for 24 hr. Doubling time was calculated with the R package “growthcurver” (Sprouffske and Wagner 2016), normalized to the strain containing the wild-type tRNA and plotted against the percent mistranslation at all proline codons determined through whole proteome mass spectrometry. (C) Yeast strains containing tti2-L187P (CY7020) and either wild-type tRNASer, tRNASerUGG-G9A or tRNASerUGG-G26A were grown to saturation in media lacking uracil and spotted in 10-fold serial dilutions on media lacking uracil or YPD containing 5% ethanol.