Figure 3.
Mutations to the discriminator base of tRNASerUGG allow mistranslation. (A) Structure of tRNASerUGG-G73C and -G73A. (B) Wild-type strain (BY4742) containing either wild-type tRNASerUGA, tRNASerUGG-G26A or tRNASerUGG-G73C were grown to saturation in media lacking uracil and spotted in 10-fold serial dilutions on media lacking uracil. (C) Wild-type strains containing one of the tRNAs described in B and a fluorescence heat shock reporter were grown in media lacking uracil and histidine. Cells were diluted 1:20 in the same media and grown for 6 hr. Cell densities were normalized and fluorescence measured. Each point represents one biological replicate.