Figure 6.
Growth of tRNASerUGG variants at different temperatures. (A) Wild-type strains containing a mistranslating tRNASerUGG variant were grown to saturation in media lacking uracil. Optical density was normalized and cultures were diluted 1:100, spotted on media lacking uracil, and grown at either 18° for 4 days or 30° for 2 days. Pixel intensity was measured for each spot. Each value is the average of three biological replicates. (B) Five representative temperature curves from the data presented in A. Strains were grown at 18° for 4 days, 24° for 3 days, or 30° or 37° for 2 days. Each point is the average of three biological replicates and error bars indicate 1 SD.