Table 1. Mutations to tRNASerUGG allowing nontoxic levels of mistranslation.
| Mutationa | Doubling time (min) | Heat-shock induction (fold change) |
|---|---|---|
| tRNASerUGA | 65.5 | 1.0 |
| A20bG | 67.4 | 1.6 |
| G9A | 68.3 | 1.2 |
| U44G | 71.6 | 2.4 |
| C5U | 75.5 | 1.9 |
| Ce21Ub | 77.7 | 1.4 |
| A4G | 77.9 | 1.7 |
| G45A | 79.4 | 2.3 |
| A38G | 80.4 | 2.2 |
| A29G | 81.6 | 3.3 |
| U19C | 85.3 | 3.4 |
| A59G | 85.4 | 2.7 |
| G26A | 88.9 | 3.2 |
| U33G | 98.0 | 2.8 |
| U51C | 101 | 3.8 |
| G17A | 102 | 2.5 |
| C12U | 105 | 2.8 |
| Ge23Ab | 105 | 4.2 |
| G15A | 107 | 2.9 |
| C48U | 109 | 6.5 |
| U60C | 113 | 1.7 |
| C40U | 116 | 3.8 |
| U39G | 129 | 2.4 |
a
With the exception of the wild-type tRNASerUGA, all tRNAs have the UGG anticodon.
b
Refers to the extended variable arm, as numbered by Sprinzl et al. (1998).