Table 3.

Primers and probes sequences were used for Lactobacillus, Escherichia coli and total bacteria analysis

Primers	Nucleotide sequence, 5'-3'	Annealing temperature, °C	Product size, bp
Total bacteria		60	200
Forward	ACTCCTACGGGAGGCAGCAG
Reverse	ATTACCGCGGCTGCTGG
Lactobacillus		60	126
Forward	GAGGCAGCAGTAGGGAATCTTC
Reverse	CAACAGTTACTCTGACACCCGTTCTTC
Probe	AAGAAGGGTTTCGGCTCGTAAAACTCTGTT
Escherichia coli		60	96
Forward	CATGCCGCGTGTATGAAGAA
Reverse	CGGGTAACGTCAATGAGCAAA
Probe	AGGTATTAACTTTACTCCCTTCCTC