Table 4.

Primer sequences were used for real-time PCR¹

Gene	Primer sequences, 5′–3′	Product size, bp	Annealing temperature, °C	GenBank accession no.
TLR2	Forward: AGTTGAAGACGCTCCCAGATG	170	62.5	AB 085935.1
	Reverse: GAAGGACAGGAAGTCACAGGA
TLR4	Forward: TCAGTTCTCACCTTCCTCCTG	166	59.5	GQ 503242.1
	Reverse: GTTCATTCCTCACCCAGTCTTC
NF-κB p65	Forward: TACGACCTGAATGCTGTGCG	130	60.0	NM 001114281
	Reverse: TGAGCTCTGCAGTGTTGGG
PPAR-γ	Forward: ATCCCATTCCCGAGAGCTGA	170	59.8	NM 214379
	Reverse: ATTGCCATGAGGGAGTTGGA
IL-8	Forward: TGAGAGTGATTGAGAGTGGA	155	58.8	NM 213867
	Reverse: CTGCTGTTGTTGTTGCTTCTC
β-actin	Forward: TCTGGCACCACACCTTCT	114	56.0	DQ 178122
	Reverse: TGATCTGGGTCATCTTCTCAC

¹TLR = toll-like receptor; IL = interleukin; NF-κB p65 = nuclear factor-κB p65; PPAR-γ = peroxisome proliferator-activated receptor gamma.