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Relationship between CH4 emissions (CH4 mL−1) and total methanogens (a), Firmicutes (b), Bacteroidetes (c), Methanobrevibacter sp. AbM4 (d), total protozoa (e), and Firmicutes/Bacteroidetes (F/B) ratio (f) in an in vitro rumen incubation with pooled rumen fluid obtained from 6 cannulated steers.
