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. 2019 Oct 28;7:247. doi: 10.3389/fcell.2019.00247

FIGURE 3.

FIGURE 3

The phenotype of pat-3(Y804E) transgenic rescue animals. (A) pat-3(+) body wall muscle stained with rhodamine-phalloidin shows normal muscle filaments. (B) pat-3(Y804E) transgenic animal has organized muscle filament patterns; each filament is positioned in straight lines. (C) Monoclonal antibody MH25 stains PAT-3 protein localized in the muscles of pat-3(+) and appeared with a regular distribution. (D) pat-3(Y804E) muscles showed regular dense bodies and M-line patterns. (E) pat-3(+) hermaphrodite tail. (F) The hermaphrodite tail has an abnormal shape and a protrusion in the base of the tail posterior to the anus (arrow). (G) pat-3(+) male tail with normal spicule indicated (arrow). (H) pat-3(Y804E) male tail appears abnormal with fused rays and protruding spicule (arrow). (I) pat-3(Y804E) gonad shows ectopic sperm (arrows) in the posterior body cavity near the end; in addition, panel (J), him-4(e1267) gonad also shows a similar defect (arrows) to panel (I). Bars = 50 μm.