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. 2019 Oct 31;12:9005–9016. doi: 10.2147/OTT.S213059

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© 2019 Wei et al.

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miR-503 directly targets PDK1 by binding its 3ʹ-UTR and regulating PI3K/AKT pathway. (A) A putative miRNA-503 target site in the 3ʹ-UTR of PDK1 mRNA was predicted by using bioinformatics analysis. (B) Wild-type (PDK1-WT) or mutant (PDK1-Mut) luciferase reporter and/or miRNA-503 mimic were co-transfected into HEK293T cells to measure the luciferase activity. (C) The expression level of PDK1 mRNA in 42 NSCLC tissues and adjacent tissues was measured by qRT-PCR. (D) Negative correlation between miR-503 and PDK1 mRNA in NSCLC tissues. (E) The expression level PDK1 protein and PI3K/AKT proteins in A549 cells or PC9 cells was measured by Western blotting. *P<0 0.05; **P<0 0.01 compared with the indicated controls.