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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Trends Cell Biol. 2019 Oct 6;29(11):901–911. doi: 10.1016/j.tcb.2019.08.006

Figure I for Text Box 1. Strategies for imaging and quantify microtubule transport and dynamics in neurons.

Figure I for Text Box 1.

Left Panel. Neurons expressing GFP-tubulin. Photobleaching of a small region in the axons allows to visualize movement of non-bleached microtubules through the photobleached region.

Middle Panel. Neurons expressing tubulin fused to a photoconvertible protein EOS3.2 display microtubules in green. Photoconversion of a small region of EOS-labeled microtubules with UV light creates red marks on microtubules. Photoconverted microtubules can be easily tracked and quantified in the red channel.

Right Panel. Neurons are labeled with a mitochondria-specific dye. Movement of microtubules can be tracked indirectly by observing the position of mitochondria docked on microtubules.