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. 2019 Oct 31;52(10):601–606. doi: 10.5483/BMBRep.2019.52.10.272

Fig. 3.

Fig. 3

Mitochondria mis-targeting of PRMT8 mutants. (A) Mitochondrial localization of PRMT8 mutants. PRMT8-GFP, PRMT8(N270)-GFP, and PRMT8(N20)-GFP co-localized with Tom20-mRFP, a mitochondria marker, in HEK293T cells. Scale bar, 20 μm. (B) Mitochondrial luminal localization of PRMT8(N20)-GFP in HEK293T cells. PRMT8(N20)-GFP co-localized with Tom20-mRFP, a mitochondrial outer membrane marker. The yellow line in the confocal fluorescence images indicates the paths along which the fluorescence intensities of the corresponding images were plotted to the bottom [red line: Tom20-mRFP, green line: PRMT8(N20)-GFP]. Scale bar, 20 μm. (C) N-terminal 15 amino acid sequences of PRMT8 within the amino acids. +, basic amino acids; H, hydroxylated amino acids; O, hydrophobic amino acids; mG, myristoylated Gly. (D) Cellular localization of PRMT8(N20/G2A)-GFP or PRMT8(N20/K4,15A,R6,13,14A)-GFP. Mitochondria are stained with MitoTracker, a mitochondria marker. The yellow line in the confocal fluorescence images indicates the paths along which the fluorescence intensities of the corresponding images were plotted to the bottom [red line: MitoTracker; green line: PRMT8(N20/K4,15A,R6,13,14A)-GFP]. Scale bar, 20 μm.