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. Author manuscript; available in PMC: 2020 Jul 9.
Published in final edited form as: Methods Enzymol. 2019 Jul 9;628:1–17. doi: 10.1016/bs.mie.2019.06.013

Figure 2. Dual-color LLSM imaging of metabolic condensates in live cells.

Figure 2.

(A) Dual color calibration with TetraSpek™ microspheres (200 nm in diameter) that are embedded in polyacrylamide gel. The centroids of red and green images are overlapped with subvoxel precision. Calibration factors are applied to dual-color data. (B, C and D) A representative image shows the spatial distribution of glucosomes (green, hPFKL-mEGFP) and mitochondria (red, MitoTracker Red CMXRos, Cat# M7512) in a Hs578T cell. The indicated region of interest (a white box) is enlarged for clarification. Scale bars, 10 um.