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. 2019 Nov 4;14(11):e0224628. doi: 10.1371/journal.pone.0224628

Fig 3. The effect of RA-stimulated ERK1/2 activity on the expressions of Stra8 and meiotic marker genes in XX germ cells.

Fig 3

(A) Female gonads at E12.5 were cultured with 50 μM U0126 (U0126) or without treatment (control) for 24 or 48h. After culture, germ cells were collected to analysis the transcript expressions of Stra8 and meiotic markers (Rec8, Spo11, Dmc1 and Sycp3). Results were normalized to the β-actin transcript expression. All expression values were calculated relative to control levels set at 1.0. Data represent the mean ± SEM (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control. (B) Sorted XX germ cells at E12.5 were cultured under four different conditions (control, RA, RA+U0126, U0126) for 24 and 48h. After culture, the cells were subjected to qPCR analysis for Stra8 and meiotic markers (Rec8 and Spo11). Each gene expressions were normalized to the β-actin expression. All expression values were calculated relative to control levels set at 1.0. Data represent the mean ± SEM (n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control; # p < 0.05, ### p < 0.001 vs. RA.