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. 2019 Nov 4;14(11):e0224628. doi: 10.1371/journal.pone.0224628

Fig 6. Inhibiting ERK1/2 activity suppresses RA-induced STRA8 protein expression and meiotic initiation in XY germ cells.

Fig 6

Isolated XY germ cells at E13.5 were cultured under four conditions (control, RA, RA+U0126, U0126) for 48h. (A) The cultured cells were immunostained with the anti-STRA8 antibody and counterstained with Hoechst33342 (blue). (B) The ratio (%) of STRA8-positive cells was estimated in each condition. *** p < 0.001 vs. control; ### p < 0.001 vs. RA. (C) The cultured germ cells were immunostained with the anti-γH2AX antibody (red) and counterstained with Hoechst33342 (blue). (D) The ratio (%) of γH2AX-positive cells was estimated in each condition. *** p < 0.001 vs. control; ### p < 0.001 vs. RA.