Fig 5. Phylogenetic tree of species of the six insect orders based on multiple AA sequence alignment in COI barcode.

The tree also indicates substitutions of the six varied AAs (at positions 15, 21, 38, 41, 42 and 57) within the distance of < 4Å from heme 515. Different colors are given to substitutions at different positions, while same colors for substitutions at the same position. AA sequence of Odonata was used for comparison.