Fig 7. Evaluation of cytokine secretion.

Two weeks after the third immunization, animals were euthanized and three spleens from each group were homogenized, pooled and cultured as described in material and methods section. Supernatants were assayed in duplicate by Luminex and the results are shown as net values after subtractions of the cytokine levels obtained with medium only. All samples responded with very high levels of all cytokines under ConA stimulation. Bars represent the average result of duplicate assays. (A) Interferon-gamma; (B) IL-4; (C) IL-5; (D) IL-10; (E) IL-17.