Fig. 7.

Knockdown of CST1 suppresses proliferation in breast cancer cells. a Knockdown of CST1 resulted in reduced CST1 protein expression in BT-474 and MDA-MB-468 cell lines. b Cell proliferation after CST1 knockdown in BT-474 cells was measured by MTT assay. c Cell proliferation after CST1 knockdown in MDA-MB-468 cells was measured by MTT assay. d Cell colony formation was determined in BT-474 and MDA-MB-468 cells transfected with NC- or CST1-targeting siRNAs. e The number of colonies after knockdown of CST1 in BT-474 and MDA-MB-468 cells. f The representative pictures of proliferation-related proteins after knockdown of CST1, as determined by western blotting. Results are expressed as means ± SD (error bars). *p < 0.05, **p < 0.01, and ***p < 0.001 compared to siNC