Figure 4. Spectral characterisation of FLuc mutants with LH₂ and iLH₂ in vivo.

The normalised spectra, and a plot of average radiance, for each FLuc mutant when expressed in the Raji B lymphoma cell line engrafted in each tumour model when imaged with LH₂ is shown, (a) subcutaneous (b) systemic and (c) intracranial, and when the same animals were imaged with iLH₂ is shown, (d) subcutaneous (e) systemic and (f) intracranial. Subcutaneous (n = 9), systemic (n = 10), intracranial (n = 7). T test used to establish statistical significance comparing average radiance values (systemic model when imaged with LH₂p=0.0224).