Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Gene (Photinus pyralis)	FLuc	this paper	N/A	Amino acid changes from ref 21 and 23, codon optimised for mammalian expression for use in this paper
Strain, strain background (mouse, male)	NSG	Jax mouse strain (Charles River)	NOD.Cg-Prkdcscid Il2rgtm1WjI/SzJ	N/A
Cell line (Human, male)	Raji B lymphoma	ATCC CCL-86	N/A	Mycoplasma tested by GATC (Eurofins Genomics)
Antibody	Anti-human CD34-PE	Biolegend	Clone 581, RRID: AB_1731862	(1:20)
Antibody	Anti-human CD271-APC	Biolegend	Clone ME20.4, RRID: AB_10645515	(1:20)
Chemical compound, dye	Viability APC eFluoro780	eBioscience	N/A	(1:1000)
Antibody	Anti-mouse/human CD11b PerCP/Cy5.5	Biolegend	Clone M1/70, RRID: AB_893232	(1:20)
Antibody	Anti-human CD19 FITC	eBioscience	Clone HIB19, RRID: AB_10669461	(1:20)
Antibody	Anti-human CD20 eFLuor 450	eBioscience	Clone 2H7, RRID: AB_1633384	(1:20)
Chemical compound, drug	Luciferin	Regis technologies	N/A	N/A
Software, algorithm	Living Image	Perkin Elmer	N/A	N/A
Software, algorithm	Prism	Graphpad	N/A	N/A
Software, algorithm	Excel	Microsoft	N/A	N/A
Software, algorithm	Flow Jo	Tree Star Inc (Oregon, USA)	N/A	N/A
Software, algorithm	PyMOL software	Schrodinger	N/A	N/A
Software, algorithm	CrysalisPro	Agilent Technologies	N/A	N/A
Software, algorithm	BD FACSDIVA	BD biosciences	N/A	N/A
Other
Chemical compound, drug	infraluciferin	Anderson, J.C.; Grounds, H.; Jathoul, A.P.; Murray, J.A.H.; Pacman, S.J.; Tisi, L. RSC Advances 2017, 7, 3975–82	N/A	Prepared by JC Anderson laboratory
Chemical compound, drug	iDLSA	this paper	N/A	Prepared by JC Anderson laboratory, seebelow and data set at https://doi.org/10.5061/dryad.3j9kd51cs.
Chemical compound, drug	CycLuc1	Merck Millipore	N/A	N/A
Chemical compound, drug	Aka-Lumine-HCL	Wako Pure Chemical Industries	N/A	N/A