Fig 2. Schematical representation of the cloning strategy to construct the DNA plasmids used throughout this work.
Three levels of constructions were created (A), (B), (C). Level (A) deals with the DNA products generated either by PCR using the appropriate primers or by direct synthesis of codon optimized (for Escherichia coli expression) DNA of the depicted organism. Level (B) consists of single modules into Dual expression vectors (Duet). Level (C) contains the dual gene expression vectors. Non-arrowed lines indicate the appropriately named restriction sites. Letter symbols correspond to suitable sequences: PT7, T7 Promoter; O, Lac Operator; T, Termination, E.c., E. coli.