Table 2. Metabolically engineered bacterial plasmids (see Table 1 for gene details) and strains constructed or used in this work.

Plasmid name	Genotype
pRSF-TYR	RSF ori kan lacI T7prom-RsTYR-T7term
pRSF-AAS	RSF ori kan lacI T7prom-PcAAS-T7term
pRSF-TYR-AAS	RSF ori kan lacI T7prom-PcAAS-T7prom-RsTYR-T7term
pCDF-ALR-K	CDF ori aadA lacI T7prom-yahK-T7term
pCDF-ALR-D	CDF ori aadA lacI T7prom-yqhD-T7term
pS4	BBR1 ori cat Plac-UV5-aroE-aroD-aroBOPT PLtetO-1-aroG*-ppsA-tktA dbl term [29]
pY3	p15a ori bla Plac-UV5-tyrB-tyrA*-aroC T1 term Ptrc-aroA-aroL dbl term [29]
Strain name	Genotype
DH10B	F- endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ-
BL21(DE3) [subsequently referred to as BL21]	F−ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lac-UV5-T7 gene 1 ind1 sam7 nin5])
HMS174(DE3) [subsequently referred to as HMS174]	F−recA1 hsdR(rK12– mK12+) (DE3) (RifR)
BL21-pRSF-TYR	BL21 RSF ori kan lacI T7prom-RsTYR-T7term
HMS174-pRSF-TYR	HMS174 RSF ori kan lacI T7prom-RsTYR-T7term
BL21-pRSF-AAS	BL21 RSF ori kan lacI T7prom-PcAAS-T7term
HMS174-pRSF-AAS	HMS174 RSF ori kan lacI T7prom-PcAAS-T7term
BL21-pRSF-TYR-AAS	BL21 RSF ori kan lacI T7prom-PcAAS-T7prom-RsTYR-T7term
BL21- TyrOP-HTOP	BL21 [BBR1 ori cat Plac-UV5-aroE-aroD-aroBOPT PLtetO-1-aroG*-ppsA-tktA dbl term] [p15a ori bla Plac-UV5-tyrB-tyrA*-aroC T1 term Ptrc-aroA-aroL dbl term] [RSF ori kan lacI T7prom-PcAAS-T7prom-RsTYR-T7term]
HMS174-TyrOP-HTOP	HMS174 [BBR1 ori cat Plac-UV5-aroE-aroD-aroBOPT PLtetO-1-aroG*-ppsA-tktA dbl term] [p15a ori bla Plac-UV5-tyrB-tyrA*-aroC T1 term Ptrc-aroA-aroL dbl term] [RSF ori kan lacI T7prom-PcAAS-T7prom-RsTYR-T7term]
BL21-TyrOP-HTOP-ALR-K	BL21 [BBR1 ori cat Plac-UV5-aroE-aroD-aroBOPT PLtetO-1-aroG*-ppsA-tktA dbl term] [p15a ori bla Plac-UV5-tyrB-tyrA*-aroC T1 term Ptrc-aroA-aroL dbl term] [RSF ori kan lacI T7prom-PcAAS-T7prom-RsTYR-T7term] [CDF ori aadA lacI T7prom-yahK-T7term]
HMS174- TyrOP-HTOP-ALR-K	HMS174 [BBR1 ori cat Plac-UV5-aroE-aroD-aroBOPT PLtetO-1-aroG*-ppsA-tktA dbl term] [p15a ori bla Plac-UV5-tyrB-tyrA*-aroC T1 term Ptrc-aroA-aroL dbl term] [RSF ori kan lacI T7prom-PcAAS-T7prom-RsTYR-T7term] [CDF ori aadA lacI T7prom-yahK-T7term]
BL21-TyrOP-HTOP-ALR-D	BL21 [BBR1 ori cat Plac-UV5-aroE-aroD-aroBOPT PLtetO-1-aroG*-ppsA-tktA dbl term] [p15a ori bla Plac-UV5-tyrB-tyrA*-aroC T1 term Ptrc-aroA-aroL dbl term] [RSF ori kan lacI T7prom-PcAAS-T7prom-RsTYR-T7term] [CDF ori aadA lacI T7prom-yqhD-T7term]
HMS174- TyrOP-HTOP-ALR-D	HMS174 [BBR1 ori cat Plac-UV5-aroE-aroD-aroBOPT PLtetO-1-aroG*-ppsA-tktA dbl term] [p15a ori bla Plac-UV5-tyrB-tyrA*-aroC T1 term Ptrc-aroA-aroL dbl term] [RSF ori kan lacI T7prom-PcAAS-T7prom-RsTYR-T7term] [CDF ori aadA lacI T7prom-yqhD-T7term]

The asterisks in aroG* and tyrA* refer to the feedback-resistant variants of aroG and tyrA, respectively [29]. OP designation stands either for a tyrosine (Fig 1A) or a hydroxytyrosol (HT, Fig 1B) overproducer strain. OPT designation stands for codon optimized version.